%0 Journal Article
%A LIU Zhi-Xin*
%A LUO Zhi-Wen
%A SUN Yu-Juan
%A YU Nai-Tong
%A ZHANG Yu-Liang
%T Cloning and Analysis of the cat Associated Genes of <em>Hevea brasiliensis</em> and Banana in Hainan
%D 2012
%R 10.7525/j.issn.1673-5102.2012.01.009
%J Bulletin of Botanical Research
%P 47-53
%V 32
%N 1
%X According to the existence of plant cat genes in GenBank, we designed a pair of degenerate primers, and amplified cat genes of banana, <em>Hevea brasiliensis</em>, yellow lantern pepper, pineapple, sugar cane, papaya from Hainan island. Ultimately, we cloned the rubber <em>cat</em>-1 and banana <em>cat</em>-2 genes, while the others not. Sequence analysis showed that open reading frame (ORF) of rubber <em>cat</em>-1 and banana <em>cat</em>-2 genes were both 1 479 bp, and encoded 492 amino acids. GenBank accession number were HQ660587 and HQ660588. Using bioinformatics software, the tertiary structures of rubber&rsquo;s CAT-1 and banana&rsquo;s CAT-2 proteins were predicated, which were similar to Exiguobacteriu Oxidotolerans (PDB code：2j2mA0) and Pseudomonas Syringae (PDB code：1m7sA), respectively. Phylogenetic analysis indicated that Hainan <em>H.brasiliensis</em> CAT-1 possessed the highest homology with Ricinus communis CAT-1 (XP_002521709.1), while banana CAT-2 was the highest homology with Elaeis guineensis CAT-2 (ACF06566.1).
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2012.01.009