%0 Journal Article
%A $authorName.trim()
%A $authorName.trim()
%A *
%A GONG Xian-Feng
%A JIANG Shou-Gang
%A XU Chao
%A ZU Yuan-Gang
%T Nano-paclitaxel Transport by Human Intestinal Epithelial Caco-2 Cells
%D 2013
%R 10.7525/j.issn.1673-5102.2013.03.014
%J Bulletin of Botanical Research
%P 339-345
%V 33
%N 3
%X Many studies had proved that oral administration of paclitaxel was poorly absorbed and easy to cause hypersensitivity reactions. For this reason, we produced a new nano-paclitaxel by paclitaxel granule wrapped with mannitol and Human Serum Albumin (HSA), which had better water solubility and lower systemic toxicity. In order to research the absorption of nano-paclitaxel after oral administration, the transepithelial flux of nano-paclitaxel was studied using the human colonic cell line Caco-2 cells. The flux of nano-paclitaxel (at a concentration range of 0.5-20 &mu;mol&middot;L<sup>-1</sup>) across the Caco-2 cell layer was linear with time for up to 3 h. The apparent permeability coefficient (<em>P</em><sub>app</sub>) for the apical to basolateral transport of nano-paclitaxel (20.9&plusmn;2.1&times;10<sup>-6 </sup>cm&middot;s<sup>-1</sup>; N=3) and the apparent maximal concentration (K<sub>M</sub>) of the active efflux component (14.00 &mu;mol&middot;L<sup>-1</sup>) were relatively high, suggesting that nano-paclitaxel may have a better absorption rate in the intestinal tract.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2013.03.014