%0 Journal Article
%A LÜFeng-Xia;GUO Zhao-Kui;YAN Pei-Qiang;WAN Xiu-Qing;PAN Yong-Ming
%T Construction of TMV-replicase Gene-targeted Vectoer for RNA Interference
%D 2008
%R 10.7525/j.issn.1673-5102.2008.03.014
%J Bulletin of Botanical Research
%P 310-314
%V 28
%N 3
%X The TMV-replicase gene was regarded target-directed sequence for RNA interference in this paper. The target sequence was linked to pMD18-T Simple Vector and replicated in <em>E.coli</em> DH 5&alpha;. after obtained by RT-PCR reaction. The pMD18-T Simple vector (which contains target sequence) was digested restrictively by double-endonuclease, after purifying the digested product was inserted into pUCCRNAi vector by the counterrepeat way. Then the counterrepeated structure of target sequence was inserted into pC2300-35s-OCS expression vector after enzyme disgested. The recombination plamid was transferred into <em>Agrobacterium tumefaciens</em> which only contained assistant plasmid in order to construct double-plasmid expression vector. With PCR reaction and restriction endonuclease digestion, the target gene sequence was comfirmed in engineering-bacterium.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2008.03.014