%0 Journal Article
%A Chu HUANG
%A Huizhen WANG
%A Meile SUN
%A Mingyue HE
%A Yanhong CHEN
%T Silicon-mediated Regulation on Polysaccharides Synthesis of <i>Codonopsis pilosula</i> under Drought Stress Based on EMP and TCA cycle Pathways
%D 2025
%R 10.7525/j.issn.1673-5102.2025.01.011
%J Bulletin of Botanical Research
%P 98-110
%V 45
%N 1
%X <p>In recent years， frequent extreme weather and uneven distribution of rain have caused water shortage in the key growth period of <i>Codonopsis pilosula</i>， leading to declines in quality and yield. To solve this problem， this study used <i>Codonopsis pilosula</i> as the object， investigated the regulatory effects of <i>C. pilosula</i> polysaccharides（CPPs） synthesis under drought stress by silicon<italic/>addition based on glycolysis （EMP） and tricarboxylic acid cycle（TCA）. Three groups were set up： water（CK）， drought by 15% PEG-6000（D） and drought by 15% PEG-6000 after silicon treatment（Si+D）. In Si+D group， 200 mL of 2 mmol?L<sup>-1</sup> potassium silicate solution was applied to each basin on the 7th day before drought treatment. EMP and TCA-related substances， enzymes activity and key genes expression were measured at 7， 14 and 21 days respectively after drought treatment. Meanwhile， the contents of CPPs and its biosynthesis-related substances were measured to analyze comprehensively the regulation of CPPs synthesis by silicon under drought stress. The results showed that： （1） Drought stress weakened the content of photosynthetic pigment of <i>C. pilosula</i>， accelerated the consumption of sucrose， fructose and glucose， and enhanced the energy consumption in EMP and TCA pathways for the synthesis and accumulation of starch and polysaccharide， and there was a mutual fluctuation of yield and quality. （2） The silicon addition enhanced the content of relative leaf water of <i>C. pilosula</i>， inhibited the content of photosynthetic pigments， increased the sucrose and fructose contents， decreased the enzymes activity related to starch synthesis and increased the enzymes activity related to starch decomposition， resulting in the decrease of starch and polysaccharide contents， but the overall yield of <i>C. pilosula</i> were increased. Silicon addition promoted the EMP pathway of <i>C. pilosula</i> under drought stress， prevented the TCA cycle process， and made pyruvic acid， the end product of EMP， synthesize into citric acid and its accumulation. The application of silicon might affect the photosynthesis of <i>C. pilosula</i>， regulate the energy generation and distribution of EMP and TCA pathways， enhance the ability of <i>C. pilosula</i> to resist drought， and play a positive role in the synthesis of polysaccharide and the quality and yield of <i>C. pilosula</i>. However， with the extension of drought stress time， the regulation effect was weakened gradually.</p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2025.01.011