%0 Journal Article
%A Haoran WANG
%A Huiyu LI
%A Jinxia DU
%A Lianfei ZHANG
%A Tingting SHEN
%A Yiping LIN
%T Construction of Suppression Expression Vector and Genetic Transformation of <i>BpSPL9</i> gene from <i>Betula platyphylla</i>
%D 2023
%R 10.7525/j.issn.1673-5102.2023.01.004
%J Bulletin of Botanical Research
%P 30-35
%V 43
%N 1
%X <p>To clarify the function of <i>SPL</i> gene in growth and development of <i>Betula platyphylla</i>，the <i>BpSPL9</i> gene was cloned， and the expression vector of <i>BpSPL9</i> was constructed by CREST technology， and the genetic transformation was carried out by <i>Agrobacterium</i> mediated method， and the phenotype of the transgenic lines was observed， respectively. The results showed that the transgenic lines with <i>BpSPL9</i> suppressed were successfully obtained. The height of transgenic lines was significantly lower than that of non-transgenic lines， with shorter node spacing and smaller leaf area. The contents of 6-BA and IAA in transgenic lines were lower than those of the non-transgenic lines. It is speculated that <i>BpSPL9</i> gene participated in plant growth and development by affecting the synthesis of auxin and cell division.</p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2023.01.004