%0 Journal Article
%A Yi-Lan CHEN
%A Hai-Min LIAO
%A Zheng-Feng WANG
%A Xiang-Xun HUANG
%A Dong-Ming LIU
%T Chromosome Preparation and Karyotype Analysis of <i>Cordia subcordata</i>
%D 2021
%R 10.7525/j.issn.1673-5102.2021.05.006
%J Bulletin of Botanical Research
%P 684-689
%V 41
%N 5
%X <p>In order to understand the genetic background of<i> Cordia subcordata</i>， we used the root tips as materials to investigate optimal sampling and pretreatment conditions， and the cells with better chromosome dispersion were selected for karyotype analysis. The results showed that：（1）The best sampling time of <i>C. subcordata</i> was from 9：30-10：00 am and from 14：00-14：30 pm and the optimal pretreatment was immersing the root tips in saturated p-dichlorobenzene for 2 hours； （2）<i>C. subcordata</i> was diploid， with 32 chromosomes， totally 16 pairs. The karyotype formula was 2n=2X=32=32m， and the karyotype was “1A”. The absolute length variation range of chromosome was 0.38-0.69 μm， and the relative length（%） varied from 4.48-8.24. The relative length composition was 2n=2L+14M1+14M2+2S. The karyotype asymmetry coefficient（As.K%） was 58.40%. These results would provide references for chromosome preparation and karyotype analysis， and laid a foundation for cytogenetic and epigenetic studies on <i>C. subcordata</i>.</p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2021.05.006