%0 Journal Article
%A Hua-Feng CHEN
%A Li-Feng WANG
%A Song-Le FAN
%T Structural and Functional Analysis of Gibberellin Signaling Transduction Pathway Gene<i> HbRGA1</i> in Rubber Tree
%D 2021
%R 10.7525/j.issn.1673-5102.2021.04.008
%J Bulletin of Botanical Research
%P 531-539
%V 41
%N 4
%X <p>DELLA protein is a negative regulator of gibberellin signaling pathway， which inhibits plant growth and development. Analyzing the structure and function of DELLA family members will be helpful to elucidate the mechanism by which the DELLA protein regulates the growth and development of rubber trees. In this study， the full-length cDNA sequence of <i>HbRGA1</i> was cloned from the leaves of rubber tree cultivar Reyan73397. <i>HbRGA1</i> is 2 136 bp long， contains 1 839 bp ORF， and encodes 613 amino acids. The HbRGA1 protein sequence contains DELLA and GRAS superfamily conserved domains， which are 82.5% like the DELLA genes of poplar， cassava and rubber trees. qRT-PCR analysis showed that the expression level of <i>HbRGA1 </i>was high in the leaf of rubber tree， but very low in the bark and latex. The expression of <i>HbRGA1</i> in leaves was significantly up-regulated by gibberellin and abscisic acid spraying. These results indicated that HbRGA1 was closely related to gibberellin and other hormone signals in rubber trees， which laid a good foundation for further study of HbRGA1’s structure and function in the growth and development of rubber trees.</p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2021.04.008