%0 Journal Article
%A HOU Zheng
%A WANG Chao
%A WANG Yan-Min
%A WANG Yu-Cheng
%A ZHANG Yue
%A ZHAO Xin
%T Cloning and Expression Analysis of S-Adenosyl-L-Methionine Synthetase(<i>ThSAMS</i>) Gene from <i>Tamarix hispida</i>
%D 2019
%R 10.7525/j.issn.1673-5102.2019.01.014
%J Bulletin of Botanical Research
%P 113-122
%V 39
%N 1
%X A full length cDNA of a S-Adenosyl-L-Methionine Synthetase gene(named <i>ThSAMS</i>) was isolated from the transcriptome cDNA librarys of <i>Tamarix hispida</i>. The open reading frame of <i>ThSAMS</i> cDNA was 1 185 bp encoding 394 amino acids. The relative molecular weight of <i>ThSAMS</i> protein was 97.85 kDa, with isoelectric point of 5.02. Multiple alignment analysis based on the amino acids indicated that <i>ThSAMS</i> had high homology to SAMS proteins from different species, and the highest similarity of 95% to the <i>Ziziphus jujuba</i> SAMS. Quantitative real-time PCR(qRT-PCR) assay revealed that <i>ThSAMS</i> gene can respond to NaCl, PEG and ABA stress, suggesting that it may be involved in salt and drought stresses in <i>T.hispida</i>. This study provided theoretical groundwork for further exploration into the function of stress tolerance of SAMS gene in plants.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2019.01.014