%0 Journal Article
%A CHENG Yu-Xiang
%A WEI Zhen
%A XIA De-An
%T Cas9/gRNA-mediated Targeted <i>PtrFLA</i>31/34 Gene Mutation in <i>Populus</i>
%D 2018
%R 10.7525/j.issn.1673-5102.2018.05.014
%J Bulletin of Botanical Research
%P 741-747
%V 38
%N 5
%X Fasciclin-like arabinogalactan proteins(FLAs) play important roles in growth and development of plants. Based on bioinformatic analysis, we identified 46 FLAs in <i>Populus trichocarpa</i>. By phylogenetic analysis, <i>Populus</i> FLA family was classified into four Sub-groups(A-D) and <i>PtrFLA</i>31 and <i>PtrFLA</i>34 were in same small branch of the tree. By semi-quantitative RT-PCR analysis, <i>PtrFLA</i>31 and <i>PtrFLA</i>34 were specifically expressed in xylem on the high transcriptional level. In order to study their functions in wood formation, we knocked out <i>PtrFLA</i>31/34 double genes via Cas9/gRNA, and obtained three <i>fla</i>31/34 double mutants.
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2018.05.014